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FaDu SN-38 concentration was significantly (p<0.05) increased and the percentage increase was 124%, when compared with irinotecan alone ( table 3). Irinotecan concentration was significantly (p<0.05) decreased and the percentage change was −60%, when compared with irinotecan alone ( table 3). In order to determine the effect of the second course treatment on intra-tumor SN-38 concentration, we studied the sensitive FaDu tumors. Panel C1 demonstrates that the second irinotecan treatment on day 14 also induces apoptotic tumor cells labeled by arrows and the combination treatment with MSC (panel C2) significantly increased the apoptosis incidence, as seen on the corresponding bar graphs. Panel B1 visualizes that the first CPT treatment on day 7 induced several apoptotic tumor cells indicated by arrows, but the combination treatment with MSC (panel B2) did not increase the apoptotic incidence significantly. Panel A1 illustrates that MSC did not change the incidence of apoptotic tumor cells, as compared to the control (panel A2). slides in randomly selected, non-necrotic fields (×400) of tumor and expressed as a percentage. Apoptotic tumor cell nuclei were counted among 300–400 tumor cell nuclei on H.E. Apoptotic cells were identified by morphology based on the characteristic nuclear fragmentation and condensation. The pictures are representative microphotographs of conventional, formalin-paraffin sections of the tumors after hematoxylin-eosin staining. Double immunohistochemical staining for endothelial cells and pericytes in tumors Quality assurance was maintained by simultaneously assaying the quality control samples prepared in bulk, prior to assay validation. The limit of quantitation for both was 2.5ng/ml. Detection was by fluorescence, with excitation at 370nm and emission at 510nm. The separation method was carried out on a Waters Nova-Pak C18 column equipped with a ♛ondapak C18 guard column, with the mobile phase consisting of 20% acetonitrile and 80% triethylamine acetate. The lactone forms of irinotecan and its active metabolite, SN-38, were measured using a validated HPLC method with fluorescence detection as described by Warner and Burke. Protein measurement was performed on the pellet using Bradford protein assay. After centrifugation of the homogenate, the supernatant was evaporated to dry, reconstituted to mobile phase and subjected to HPLC. Samples were homogenized using a Polytron tissue homogenizer (Brinkmann Instruments, Westbury, NY). Tissue samples were immediately incubated with ice-cold 5ml methanol and acetonitrile (1:1). Plasma was obtained at 4☌ immediately following the blood collection and subjected to the same solvent extraction procedure and HPLC. Tumors, blood and normal tissue samples were collected 2h after irinotecan alone or in combination. The symptoms relapsed in 20 in which the singer had to take a short break from her activities. In early 2008, Young was diagnosed with vocal nodules and had to receive medical treatment. Throughout her career, she also participated as the host for several television programs. Besides Girls' Generation, Young‘s early solo work mostly involved songs recorded for side projects and original soundtracks. The singer adopted the stage name Tiffany, the name her mother initially wanted to name her at birth. She officially debuted as a member of South Korean girl group Girls' Generation in August 2007. Young attended Korea Kent Foreign School and was trained at SM Entertainment for almost four years. She moved to South Korea three weeks later. She was spotted by a local SM Entertainment representative, auditioned, and was accepted as a trainee. Encouraged by her brother, at the age of 15, Young participated in a singing contest. She grew up in Diamond Bar, California, and was inspired by K-pop artist BoA. Her mother died when she was 12 years old, and she is estranged from her father. Her family consists of her father, an older sister, and an older brother. Tiffany Young was born Stephanie Young Hwang on August 1, 1989, in San Francisco, California. Life and career 1989–2012: Early life and career beginnings 1.3 2017–present: Departure from SM Entertainment and solo career.1.2 2012–2016: Girls’ Generation TTS, solo debut and controversy.1.1 1989–2012: Early life and career beginnings.She joined Paradigm Talent Agency and signed with record label Transparent Arts prior to the release of her 2019 extended play Lips on Lips. She is currently based in Los Angeles for her solo career. Although Young remains a member of Girls' Generation, she left SM Entertainment in October 2017. In 2016, Young became the second Girls' Generation member to debut as a soloist with the release of her first extended play I Just Wanna Dance. After two years of training, Young debuted as a member of girl group Girls' Generation (and later its subgroup Girls' Generation-TTS) in August 2007, which went on to become one of the best-selling artists in South Korea and one of South Korea's most popular girl groups worldwide. Born and raised in California, she was discovered by South Korean entertainment agency SM Entertainment at the age of fifteen and subsequently moved to South Korea. Stephanie Young Hwang (born August 1, 1989), known professionally as Tiffany or Tiffany Young, is an American singer-songwriter. |
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